PCR Equipment and Supplies
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Invitrogen™ M13 Reverse Primer
Oligonucleotides complementary to a DNA template are necessary to prime DNA synthesis for sequencing reactions.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix
Thermo Scientific Maxima H Minus cDNA Synthesis Master Mix, available with or without dsDNase, provides all cDNA synthesis reaction components in a convenient one-tube master mix.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | Maxima H Minus |
| Final Product Type | First-Strand cDNA |
| Quantity | Each |
| For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
| Format | Master Mix |
| Size (Final Product) | Up to 20 kb |
| Reaction Format | Premixed Components |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Applied Biosystems™ GeneAmp™ dNTP Blend (2.5 mM ea)
GeneAmp dNTPs are deoxynucleotide triphosphates dissolved in glass-distilled water and titrated to pH 7.0 with NaOH. Each vial consists of 1 mL of 2.5 mM each of dATP, dCTP, dGTP, and dTTP, yielding a 10 mM blend of dNTPs.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Form | Liquid |
| Concentration | 10 mM |
| For Use With (Application) | Standard PCR |
| Product Line | GeneAmp |
Thermo Scientific™ Phusion™ HF and GC Buffer Packs
5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.
| Quantity | 4 x 1.5 mL |
|---|---|
| For Use With (Application) | Standard PCR |
Thermo Scientific™ Maxima H Minus Double-Stranded cDNA Synthesis Kit
The Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA.
| Reverse Transcriptase | Maxima H Minus |
|---|---|
| Reaction Format | Separate Components |
| Final Product Type | Double Stranded cDNA |
| Product Type | cDNA Synthesis Kit |
| Sample Type | RNA |
| For Use With (Application) | Cloning |
| Product Line | Maxima |
Thermo Scientific™ Phusion Hot Start II High-Fidelity PCR Master Mixes
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion Hot Start II High-Fidelity DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Hot Start High-Fidelity PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Applied Biosystems™ Detection Enhancer, for real-time PCR
Formulated to improve template amplification in real-time PCR (qPCR) workflows
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Product Type | Detection Enhancer |
| For Use With (Equipment) | 7500 System |
| Sample Type | Viral Samples |
| Reaction Speed | Standard |
| For Use With (Application) | Real Time PCR (qPCR) |
| PCR Method | 1-step RT-qPCR |
| Product Line | Applied Biosystems |
| Detection Method | Primer-probe |
Thermo Scientific™ Taq Buffers (10X)
Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents.
| Physical Form | Liquid |
|---|---|
| Quantity | 4 x 1.25 mL |
| Concentration | 10X |
Invitrogen™ PureLink™ Pro 96 PCR Purification Kit
The PureLink™ Pro 96 PCR Purification Kit combines advanced silica plate extraction chemistry with an optimized 96-well plate design for manual or automated processing of PCR cleanup in as little as 20 minutes.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ AmpErase™ Uracil N-Glycosylase (UNG)
Enzyme removes any uracil incorporated into single- or double-stranded DNA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix
Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | Medium |
| Content And Storage | Store at -15°C to -25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use. |
| Form | Liquid |
| Concentration | 4X |
| For Use With (Application) | Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence |
| PCR Method | 1-step RT-qPCR |
| Multiplex Capability | Up to 5-plex |
| Detection Method | Primer-probe |
| Includes | dUTP |
| Thermostability | 8 hr., Assembled Reaction |
| Polymerase | Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start |
| Format | Single-Tube |
| Passive Reference Dye | ROX (Pre-mixed) |
| Reaction Speed | Fast or Standard |
| Inhibitor Tolerance (Simple) | High |
| Target Specificity | RNA only, Both RNA and DNA |
| Hot Start | Antibody-based |
| Shelf Life | Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt |
| Product Line | TaqPath |
Invitrogen™ Buffer Kit, RNase-free
The Invitrogen Buffer Kit provides high quality, RNase free buffers for critical RNA analysis procedures.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ RNA Control 250 with Manual
The Invitrogen RNA Control 250 provides a precise, accurate, and reproducible RNA concentration reference for use in conjunction with NanoDrop™ Spectrophotometers.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Content And Storage | RNA Control and THE RNA Storage Solution should be stored at –20°C |
|---|---|
| Format | Tube |
| Label or Dye | Unconjugated |
| Product Line | Ambion |
Invitrogen™ Platinum™ SuperFi II PCR Master Mixes
Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi II DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ dTTP Solution (100 mM)
Thermo Scientific™ dTTP (2'-deoxythymidine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.